Biofilm Producing Vibrio Species Isolated from Siloso Beach, Singapore : A Preliminary Study

This research aim to preliminarily investigate biofilm production among Vibrio species isolated from Siloso beach, Singapore. 100 μL of sea water sample collected was plated on Congo Red Agar (CRA) using spread plate method. The plates were incubated at 30°C for 24 hours. Forty – six biofilm producing black colonies with dry crystalline consistence were further transferred aseptically into freshly prepared Thiosulfate-Citrate-Bile salts-Sucrose agar (TCBS), a selective medium for isolation of Vibrio spp. All the isolates were found to grow on TCBS after 24 hours incubation at 30°C. Biofilm production in marine bacteria is of significance important to public health. The result of this preliminary study speaks volume of the health related danger in recreation water bodies.